Analysis Note

Color (visual): white to yellow to brownAppearance of substance (visual): beadsLoading (determined from the substitution of the Fmoc-Leu loaded resin): 0.30 - 0.60 mmol/gSwelling Volume (in DMF): lot specific resultThe polymer matrix is copoly styrene - 1 & DVB 100 - 200 mesh

General description

The EDANS/dabcyl fluorophore-quencher pair is one of the most commonly-used for FRET peptides, owing to excellent spectral overlap between the emission spectrum of EDANS and absorbance spectrum of dabcyl [1]. The introduction of the EDANS moiety is traditionally achieved either by coupling of a peptide fragment to EDANS in solution [1, 2] or through the use of a pre-formed derivative, such as Fmoc-Glu(EDANS)-OH [3]. Novabiochem′s EDANS resin enables for the first time the direct synthesis of C-terminally EDANS-labeled peptides by solid phase synthesis [4]. Loading of the first residue requires acylation of the resin-bound secondary amine and is, therefore, best carried out using HATU activation. Chain extension and cleavage can then be effected using standard methods.

EDANS NovaTag™ resin has been recently employed to prepare fluorescently labeled aminoalkane diphenyl phosphonate affinity probes for chymotrypsin- and elastase-like serine proteases [5].



The product number for this product was previously 04-12-3904.

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Legal Information

NOVATAG is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVASYN is a registered trademark of Merck KGaA, Darmstadt, Germany

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-12-3904

Packaging

100 mg in Glass bottle